Sample Mixturea | Retention Time | λmax | Structure of Reference Compound | ||
---|---|---|---|---|---|
RP-HPLC | NP-HPLC | ||||
min | nm | ||||
[14C]Product I + | 22.9 | 20.5 | <200 | ||
[12C]12-oxo-ETrE | 22.6 | 20.0 | <200 | ||
[14C]Product II + | 20.5 | 20.4 | <200 | ||
[12C]12-HETrE | 20.0 | 19.9 | <200 | ||
[14C]Product III + | 20.0 | 24.7 | 281.6b | ||
[12C]12-oxo-ETE | 19.5 | 24.3 | 281.6b | ||
[14C]Product IV + | 18.6 | 21.3 | 236.8 |
| |
[12C]12-HETE | 18.1 | 20.8 | 236.8 |
↵ a Purified radiolabeled products (I–IV) were mixed with unlabeled authentic standards, and the mixtures were resolved by RP- and NP-HPLC. The lag time between UV and radioactivity detectors was 0.5 ± 0.1 min
↵ b The composition of the reversed-phase gradient system was water/acetonitrile/acetic acid (24.95:74.95:0.1 v/v/v)