Protein yield (mg/g wet tissue) and the activities of citrate synthase (mU/mg of protein) of SSM and IFM following ischemia with and without amobarbital treatment
Data are expressed as mean ± S.E.M. The protein yield (milligrams of mitochondrial protein per gram wet weight of heart) and citrate synthase activities (mU/milligrams of mitochondrial protein) were not altered either by ischemia alone or by amobarbital treatment in SSM and IFM (all data, p = N.S.).
SSM | IFM | |||||
|---|---|---|---|---|---|---|
| Protein Content | Citrate Synthase Activity | Protein Content | Citrate Synthase Activity | |||
| Time control (n = 6) | 11.2 ± 1.0 | 2074 ± 124 | 9.1 ± 0.5 | 2434 ± 65 | ||
| Ischemia (n = 5) | 10.5 ± 0.7 | 1751 ± 86 | 8.6 ± 0.6 | 2379 ± 93 | ||
| 1.25 mM Amobarbital (n = 3) | 10.1 ± 1.0 | 2101 ± 135 | 8.0 ± 0.9 | 2631 ± 227 | ||
| 2.0 mM Amobarbital (n = 3) | 8.7 ± 0.6 | 2077 ± 169 | 7.9 ± 0.4 | 2690 ± 108 | ||
| 2.5 mM Amobarbital (n = 5) | 9.0 ± 0.3 | 1740 ± 124 | 8.6 ± 0.3 | 2490 ± 77 | ||
| 3.75 mM Amobarbital (n = 3) | 11.5 ± 0.7 | 1873 ± 42 | 8.5 ± 0.4 | 2632 ± 112 | ||
| 5.0 mM Amobarbital (n = 3) | 9.2 ± 0.6 | 1921 ± 90 | 7.0 ± 0.6 | 2731 ± 271 | ||