Glucuronidation activities of the recombinant UGTs 1A5 and 1A4 as well as the chimeric enzymes 1A4/5 and 1A5/4
The units are picomoles × minutes-1 × milligrams-1, and the results are the mean ± S.D. (n = 3).
4-Methylumbelliferone | Scopoletin | 4-Aminobiphenyl | 1-Hydroxypyrene | |
|---|---|---|---|---|
| UGT1A5 | 0.9 ± 0.1 | 1.1 ± 0.1 | N.D. | 96.8 ± 5.3 |
| UGT1A4 | 1.1 ± 0.1 | 1.4 ± 0.1 | 945.6 ± 32.6 | 103.8 ± 7.2 |
| UGT1A4/5 | 1.3 ± 0.1 | 1.5 ± 0.1 | N.D. | 59.5 ± 1.8 |
| UGT1A5/4 | 2.3 ± 0.1 | 6.0 ± 0.3 | 0.6 ± 0.1 | 46.4 ± 4.9 |
N.D., no detectable activity.