Primers used for site-directed mutagenesis of CYP2C9*1
Primer Name | Mutation | Primer Sequence |
|---|---|---|
| 2C9Exon3/371Forward | Arg124Gln | ATGGAAGGAGATCCAGCGTTTCTC |
| 2C9Exon3/371Reverse | GAGAAACGCTGGATCTCCTTCCAT | |
| 2C9Exon3/374Forward | Arg125His | GAGATCCGGCATTTCTCCCTCATGACG |
| 2C9Exon3/374Reverse | CGTCATGAGGGAGAAATGCCGGATCTC | |
| 2C9Exon4/485Forward | Ser162X | AACCAAGGCCTAACCCTGTGATCCCAC |
| 2C9Exon4/485Reverse | GTGGGATCACAGGGTTAGGCCTTGGTT | |
| 2C9Exon6/895Forward | Thr299Ala | GTTTGGAGCTGGGGCAGACGACAAG |
| 2C9Exon6/895Reverse | CTTGTCGTGTGTGCCCCAGTCCAAAC | |
| 2C9Exon7/1144Forward | Pro382Ser | CAGAAACTATCTCATTTCCAAGGGCACAACC |
| 2C9Exon7/1144Reverse | GGTTGTGCCCTTGGAAATGAGATAGTTTCTG | |
| 2C9Exon8/1190Forward | Asp397Ala | CCTGACTTCTGTGCTACATGCCAACAAAGAATTTCC |
| 2C9Exon8/1190Reverse | GGAAATTCTTTGTTGGCATGTAGCACAGAAGTCAGG | |
| 2C9Exon9/13262Forward | Gln454His | CCTGACCTCCATTTTACACAACTTTAACCTG |
| 2C9Exon9/13262Reverse |
| CAGGTTAAAGTTGTGTAAAATGGAGGTCAGG |