TABLE 1

Oligonucleotides for real-time PCR

Sequences are derived from GenBank accession numbers AF017079 (GAPDH), AF540788 [B₁ receptor (B₁R)], AB051422 [B₂ receptor (B₂R)], U55039 (mAPP), and NM_000902 (NEP).

mRNA Targets	Nucleotide Sequences (5′ → 3′)^a	Product Size	Final Concentration^b
		bp	μM
GAPDH-GAPDH gene	F834: ACT TCG GCA TCG TGG AAG GAC T[R]	500	0.7
	R1334: GGT CCA GGG GCT CTT ACT CCT T[R]
B₁R-BDKRB1 gene	F5: CTC CCA GAC CCT CGT GGT GT[R]	350	0.7
	R355: CCA GGA AGA TGC TGA TGA ACA GGT[R]
B₂R-BDKRB2 gene	F110: TGA ACA CGG CAC AGC AGA GAC CTG AGT TCC[R]	500	0.7
	R610: CTT CAC CAG GGC CAG GTA GCG GTC GAT G[R]
mAPP-XPNPEP2 gene	F1716: AAC AAC AGA CAT CAC CCG AA[R]	220	0.5
	R1936: CAC ACA GGA AGT TGC CG[R]
NEP-MME gene	F1246: CAG CCG AAC CTA CAA GGA GTC CAG AAA TGC[R]	599	0.7
	R1845: TGT CAT CGA AGC CAT GGG TGA TTT CGT GTC[R]

↵ a F and R indicate forward and reverse primers, respectively; numbers indicate the sequence position.
↵ b Final concentration of forward and reverse primers.