TABLE 1

Summary of PCR primers sequences for P2Y receptor

Gene
Primer Sequences (5′-3′)
Target Size
Annealing
Cycle
bp °C
P2Y1 F: CCGCCGCCTAAGTCGAG 640 52 25 cycles followed
R: GGTGTCGTAACAGGTGATGG by 20 cycles
P2Y2 F: CCAGGCCCCCGTGCTCTACTTTG 367 56 40
R: CATGTTGATGGCGTTGAGGGTGTG
P2Y4 F: CCACCTGGCATTGTCAGACACC 425 58 35
R: GAGTGACCAGGCAGGCACGC
P2Y6 F: CGCTTCCTCTTCTATGCCAACC3 65 60 35
R: CCATCCTGGCGGCACAGGCGGC
Cyclophilin F: GGTCAACCCCACCGTGTTCTTCG 566 60 25
R: GTGCTCTCCTGAGCTACAG

  • bp, base pair(s); F, forward; R, reverse.