Overexpression of Egr-1 induces ATF3 mRNA gene expression HCT-116 cells were transiently transfected as described under Materials and Methods using vector or an Egr-1 plasmid expressing full-length protein. Primers for exogenous Egr-1 recognize the Egr-1 sequence used in the overexpression plasmid, whereas primers for endogenous Egr-1 are from an untranslated mRNA region not found nor required in the overexpression plasmid and is shown for comparison. Values are expressed as mean (±S.E.M.) relative to vector-transfected cells according to real-time RT-PCR adjusted for actin.

Egr-1 Overexpression

-Fold mRNA
ATF3 3.5 ± 0.12
Exogenous Egr-1 8.4 ± 0.16
Endogenous Egr-1
0.88 ± 0.03