TABLE 1

Effects of antisera against TNF-α, IL-12, and IL-18 and their respective control sera on TNF-α-, IL-12-, and IL-18-induced mechanical hyperalgesia, respectively The rats were treated with antisera against rat TNF-α (α-TNF-α; 15 min, 50 μl i.pl.), IL-12 antibody (α-IL-12; 30 min, 10 ng in 50 μl i.pl.), IL-18 antibody (α-IL-18; 30 min, 10 μg in 50 μl i.pl.), or control sera (α-control; 15 min, 50 μl i.pl.) before TNF-α (2.5 pg in 50 μl i.pl.), IL-12 (10 ng in 50 μl i.pl.), IL-18 (40 ng in 50 μl i.pl.), or saline (50 μl i.pl.) administration, respectively. Each group represents means ± S.E.M. of five animals.

	Constant Pressure Test					Electronic Pressure-Meter Test
Group/Time	1 h	3 h	5 h	1 h	3 h	5 h
		Δ reaction time, s			Δ reaction force, g
Saline + saline	0.9 ± 0.3	1.5 ± 0.6	1.3 ± 0.5	0.7 ± 0.7	0.5 ± 0.3	0.7 ± 0.5
α-Control + saline	1.1 ± 0.5	1.6 ± 0.8	2.0 ± 0.7	1.6 ± 0.5	1.8 ± 0.9	1.9 ± 0.6
α-Control + TNF-α	9.4 ± 0.6^*	16.2 ± 0.4^*	12.6 ± 1.1^*	12.9 ± 0.8^*	17.9 ± 1.4^*	14.5 ± 1.6^*
α-TNF-α + TNF-α	2.0 ± 0.4^†	3.9 ± 1.1^†	3.5 ± 0.5^†	8.1 ± 1.9^†	8.3 ± 1.4^†	8.1 ± 0.5^†
α-Control + IL-12	15.5 ± 0.6^*	17.2 ± 0.8^*	18.6 ± 0.5^*	12.4 ± 1.7^*	18.4 ± 1.6^*	18.6 ± 1.4^*
α-IL-12 + IL-12	1.2 ± 0.6^†	1.5 ± 0.7^†	1.4 ± 0.6^†	6.8 ± 0.7^†	7.7 ± 0.8^†	8.7 ± 1.4^†
α-Control + IL-18	8.3 ± 1.4^*	16.3 ± 0.8^*	14.7 ± 0.5^*	15.4 ± 6.2^*	27.6 ± 3.8^*	22.8 ± 2.6^*
α-IL-18 + IL-18	1.2 ± 0.4^†	4.6 ± 1.1^†	1.9 ± 0.6^†	1.0 ± 0.6^†	7.3 ± 0.5^†	10.0 ± 0.9^†

↵* P < 0.05 compared with the α-control + saline group
↵† P < 0.05 compared with the α-control + respective cytokine group (one-way ANOVA followed by Bonferroni's t test)