TABLE 1

Effect of BMS-345541 on IL-1-stimulated gene expression in SW-1353 chondrosarcoma cells mRNA values normalized to GAPDH, with unstimulated controls set to a relative value of 1.0. The data represent the mean ± S.D.

Gene
Unstimulated Expression
IL-1-Stimulated Expression
No Inhibitor
0.1 μM BMS-345541
1 μM BMS-345541
10 μM BMS-345541
COX-2 1.0 ± 0.8 118 ± 18 151 ± 49 8 ± 2* 0.1 ± 0.1*
iNOS 1.0 ± 0.5 91 ± 15 127 ± 27 21 ± 3* 0.4 ± 0.2*
MMP-1 1.0 ± 0.5 22 ± 7 33 ± 12 4 ± 2* 0.4 ± 0.2*
MMP-3 1.0 ± 0.4 1928 ± 457 2500 ± 710 107 ± 21* 0.8 ± 0.4*
MMP-13 1.0 ± 0.3 65 ± 25 129 ± 59 12 ± 4* 0.9 ± 0.3*
ADAMTS-4 1.0 ± 0.4 1.0 ± 0.2 1.7 ± 0.9 0.6 ± 0.2 0.5 ± 0.2
ADAMTS-5 1.0 ± 0.3 0.9 ± 0.3 1.4 ± 0.5 0.6 ± 0.1 1.0 ± 0.3
TIMP-1 1.0 ± 0.2 1.8 ± 0.5 3.3 ± 2.3 1.1 ± 0.1** 0.6 ± 0.2*
TIMP-3
1.0 ± 0.5
0.9 ± 0.2
1.3 ± 0.3
1.1 ± 0.1
0.7 ± 0.5

  • ↵* P < 0.01 versus IL-stimulated control without inhibitor, n = 5 independent samples per condition

  • ↵** P < 0.05 versus IL-stimulated control without inhibitor, n = 5 independent samples per condition