TABLE 2

Protein expression altered by chronic morphine in hMOR-CHO cells Samples were proteolyzed and analyzed using a Micromass Q-TOF Ultima Global as described under Materials and Methods. Data were processed using ProteinLinx to generate peak list files prior to an in-house licensed Mascot search at http://biospec.nih.gov (MatrixScience Ltd.).


Spot No.

Molecular Mass

pI

Protein Identified

Sequences from Database

Up- or Down-Regulation
kDa
48 86 6.33 Junction plakoglobin (desmoplakin III) 427TLVTQNSGVEALIHAILR444 Up
583LNTIPLFVQLLYSSVENIQR602 Up
80 77 6.35 Phosphoglucomutase 1 108GVVISFDAR116
309GVLTLSFALADKIK322
5017LFGLNR506
521FEISAIR527
528DLTTGYDDSQPDKK541
Heat shock protein cognate 71 37TTPSYVAFTDTER49 Up
50LIGDAAK56
160DAGTIAGLNVLR171
172IINEPTAAAIAYGLDKK188
329SQIHDIVLVGGSTR342
91 74 5.26 Ubiquilin 222NPEISHMLNNPDIMR236 Up
237QTLELAR243
233 52 6.77 Tubulin β chain 63AILVDLEPGTMDSVR77 Up
77SGPFGQIFRPDNFVFGQSGAGNNWAK103
104GHYTEGAELVDSVLDVVR121
155IREEYPDR162
163IMNTFSVVPSPK174
253LAVNMVPFPR262
263LHFFMPGFAPLTSR276
283ALTVPELTQQVFDAK297
245 50 6.77 Aldehyde dehydrogenase E3′ 3AFEPATGR10 Up
289IGDPLLEDTR298
342 40 7.68 Sialidase 2 (N-acetyl-α-neuraminidase) 10ETLFQTGDYAYR21 Down
45TDEHADLFVLR55
244VQAQSPNSGLDFQDNQVVSK263
285ADALDVWLLYTHPTDSR301
632 18 4.87 Ribosomal protein, large P2 3VASYLLAALGGNSSPSAK21 Down




50NIEDVIAQGVGK61