Increased Dectin-1 and TLR2 expression in murine GALT cells following oral administration of water-soluble glucans
Mice were gavaged with scleroglucan, glucan phosphate, or laminarin (1 mg/mouse) at time 0. The mice were sacrificed 24 h after treatment, and GALT cells were harvested. n = 3/group; mean ± S.E.M.
Control | Laminarin | Glucan Phosphate | Scleroglucan | |
|---|---|---|---|---|
| Percentage of Dectin-positive | ||||
| Total | 38.2 ± 1.8 | 71.8 ± 9.1a | 76.9 ± 3.1a | 66.0 ± 1.6a |
| Dendritic cells (CD3+) | 91.7 ± 2.2 | 94.9 ± 2.9 | 98.3 ± 0.4 | 96.2 ± 2.0 |
| Lymphocytes (CD11+) | 70.2 ± 4.8 | 59.9 ± 20.4 | 70.5 ± 15.0 | 52.6 ± 21.1 |
| PMN+ | 95.5 ± 0.7 | 80.9 ± 7.3 | 83.6 ± 7.5 | 93.2 ± 3.3 |
| Macrophages (F4/80+) | 67.6 ± 5.2 | 90.2 ± 5.0a | 91.2 ± 4.5a | 98.7 ± 0.7a |
| Percentage of TLR2-positive | ||||
| Total | 41.8 ± 3.0 | 47.2 ± 9.5 | 52.5 ± 1.1 | 51.6 ± 5.6 |
| Dendritic cells (CD3+) | 75.3 ± 3.7 | 98.6 ± 0.82 | 98.8 ± 0.22 | 98.8 ± 0.82 |
| Lymphocytes (CD11+) | 13.7 ± 2.6 | 24.5 ± 3.8 | 36.1 ± 12.3 | 45.2 ± 24.2 |
| PMN+ | 89.7 ± 15.8 | 78.2 ± 1.0 | 70.6 ± 3.8 | 70.9 ± 8.8 |
| Macrophages (F4/80+) | 66.5 ± 10.3 | 82.3 ± 5.1 | 72.6 ± 12.7 | 81.7 ± 2.1 |
↵ a p < 0.05 versus control.