TABLE 2

Effect of PMN-depleting antiserum on types of PMNs accumulated in livers of LPS/RAN-treated rats

Rats were pretreated with either 0.25 ml of NS or NAS diluted 1:1 in sterile saline (i.v.); 16 h later, they were given 44.4 × 106 EU/kg LPS or its Veh (i.v.), and then, 2 h later, 30 mg/kg RAN or its Veh was administered (i.v.). PMN immunohistochemistry and classification and enumeration of liver PMNs were performed 6 h after RAN administration as described under Materials and Methods.

Serum Pretreatment
Treatment
Average Number of PMNs/400× HPF
Segmented
Band
Degenerate
NS Veh/Veh 0.4 ± 0.05 0 0
NAS Veh/Veh 0.2 ± 0.05 0.1 ± 0.04 0
NS LPS/RAN 16.6 ± 2.1* 1.4 ± 0.4* 6.5 ± 1.0*
NAS
LPS/RAN
8.9 ± 0.4*,**
2.2 ± 0.3*,**
2.0 ± 0.3*,**

  • ↵* Significantly different from respective Veh/Veh-treated group.

  • ↵** Significantly different from respective normal serum-treated group (p < 0.05).