TABLE 1

Effect of sEH inhibitors on the conversion of [³H]14,15-EET to 14,15-DHET by COS-7 cells The COS-7 cells were incubated initially for 30 min in 1 ml of DMEM, with or without 10 μM sEH inhibitors. After the addition of 1 μM [³H]14,15-EET to the medium, the incubation was continued for 1 h, and the radiolabeled 14,15-DHET contained in the medium was assayed by reverse-phase HPLC as described in Fig. 2. The values are results obtained from three separate cultures, expressed as mean ± S.D. The specific activity of the added [³H]14,15-EET was used to calculate the pmol amounts.