TABLE 1

Test results obtained with saline, GHB, DTT given before 200 mg/kg GHB, and with other drugs given alone in different groups of rats trained to discriminate 200 mg/kg GHB either from saline (group 1), saline and 3.2 mg/kg baclofen (group 2), or saline, 3.2 mg/kg baclofen, and 1 mg/kg diazepam (group 3) Compounds were tested in seven to eight rats of groups 1 and 3 and in five to six rats of group 2.

Discrimination Test Compound
Dose
Group 1: GHB vs. Salinea
Group 2: GHB vs. Saline or Baclofen
Group 3: GHB vs. Saline, Baclofen, or Diazepam
% GHB
Rate
% GHB
Rate
% GHB
Rate
mg/kg
Saline 0 0.0 ± 0.0 0.98 ± 0.08 0.0 ± 0.0 1.13 ± 0.04 0.1 ± 0.1 1.12 ± 0.06
GHB 200 83.1 ± 12.6 0.59 ± 0.09 92.6 ± 7.1 0.81 ± 0.10 99.7 ± 0.2 0.82 ± 0.07
DTT before 200 mg/kg GHB 1 85.4 ± 14.2 0.89 ± 0.12 80.0 ± 19.9 0.90 ± 0.10 87.3 ± 12.5 0.80 ± 0.09
3.2 71.3 ± 18.4 0.88 ± 0.12 75.1 ± 25.0 0.96 ± 0.09 50.0 ± 18.9 0.84 ± 0.05
10 50.3 ± 22.1 0.65 ± 0.11 77.3 ± 19.4 0.85 ± 0.04 50.2 ± 18.8 0.77 ± 0.04
17.8 66.7 ± 20.9 0.76 ± 0.09 60.1 ± 24.4 0.70 ± 0.06 37.8 ± 18.1 0.69 ± 0.04
32 N.D. 0.10 ± 0.10 N.D. 0.01 ± 0.00 74.4 ± 24.9 0.22 ± 0.08
Pentobarbital 1 0.0 ± 0.0 1.31 ± 0.06 0.0 ± 0.0 1.19 ± 0.14 0.0 ± 0.0 1.13 ± 0.04
3.2 0.0 ± 0.0 1.13 ± 0.07 0.0 ± 0.0 0.91 ± 0.24 0.0 ± 0.0 1.20 ± 0.07
5.6 N.T. 20.2 ± 19.9 0.83 ± 0.10 0.0 ± 0.0 1.10 ± 0.05
10 N.D. 0.04 ± 0.03 N.D. 0.26 ± 0.16 N.D. 0.27 ± 0.19
Cocaine 1 0.1 ± 0.1 1.14 ± 0.14 N.T. 14.3 ± 14.3 0.97 ± 0.05
3.2 10.5 ± 9.5 0.86 ± 0.11 0.2 ± 0.2 1.01 ± 0.12 12.5 ± 12.5 1.06 ± 0.06
10 20.0 ± 20.0 0.61 ± 0.20 0.0 ± 0.0 0.74 ± 0.21 0.1 ± 0.0 0.80 ± 0.12
17.8 0.1 ± 0.1 0.44 ± 0.19 2.5 ± 2.3 0.45 ± 0.14 0.8 ± 0.8 0.38 ± 0.14
Ketamine 0.32 12.5 ± 12.5 1.25 ± 0.06 N.T. N.T.
1 35.4 ± 17.3 1.20 ± 0.08 0.0 ± 0.0 1.13 ± 0.04 12.5 ± 12.5 1.14 ± 0.05
3.2 32.2 ± 16.3 0.90 ± 0.11 0.2 ± 0.2 0.84 ± 0.08 0.0 ± 0.0 1.06 ± 0.05
5.6 N.T. 0.1 ± 0.1 0.59 ± 0.21 0.0 ± 0.0 0.58 ± 0.19
10 N.D. 0.15 ± 0.15 N.D. 0.22 ± 0.14 0.1 ± 0.0 0.64 ± 0.15
17.8 N.T. N.D. 0.08 ± 0.08 N.D. 0.09 ± 0.08
Morphine 0.32 0.4 ± 8.1 1.17 ± 0.09 N.T. N.T.
1 37.1 ± 9.1 1.14 ± 0.08 16.6 ± 16.6 0.96 ± 0.06 14.3 ± 14.3 0.83 ± 0.14
3.2 36.9 ± 10.1 0.75 ± 0.13 16.5 ± 16.5 0.74 ± 0.06 14.3 ± 14.3 0.70 ± 0.10
5.6 N.T. 1.6 ± 1.0 0.45 ± 0.10 24.8 ± 16.2 0.58 ± 0.07
10
N.D.
0.14 ± 0.09
N.D.
0.21 ± 0.10
42.3 ± 21.4
0.28 ± 0.09

  • % GHB, mean (±1 S.E.M.) percentage of responses on the GHB-appropriate lever; N.D., not determined due to rate-decreasing effects at this dose (i.e., response rate was <20% of control in more than half the animals tested); rate, mean (±1 S.E.M.) responses per second; and N.T., not tested at this dose.

  • ↵ a From Carter et al. (2003), except the data obtained with DTT