Primers and probes used for rat CYP2B1 and CYP2B2 mRNA in TaqMan assay
GenBank (Accession No.) | Primers and Probes (Position) | Sequence 5′ to 3′ |
|---|---|---|
| CYP2B1 (M37134) | Forward primer (250–267) | GTGGGCCAAGCTGAGGAT |
| Reverse primer (352–368) | GAGAATCGCCGAAGGGC | |
| Probe (286–313) | 5′-FAM-ATCGCTGTGATTGAGCCAATCTTCAAGG-BHQ1–3′ | |
| CYP2B2 (M34452) | Forward primer (495–514) | TTCTGCGCATGGAGAAAGTG |
| Reverse primer (560–583) | GATCATGAGGTTCTCATGATGGAA | |
| Probe (518–544) | 5′-FAM-CCTGCATGGATGAGAGAGGAGAAGTCG-BHQ1–3′ | |
| GUS (NM_017015) | Forward primer (1730–1754) | AATATGTCATCGGAGAGCTCATCTG |
| Reverse primer (1810–1831) | TCTGTCGAGTGAAGATCCCCTT | |
|
| Probe (1775–1804) | 5′-FAM-CGAACCAGTCACCACTGAGAGTAACAGGAA-BHQ1–3′ |
FAM, 5-carboxyfluorescein fluorescent reporter dye; BHQ1, black hole quencher 1.