TABLE 1

Univariate analysis of effects of liver donor demographics and genotype on UGT1A6 glucuronidation, protein, and mRNA content

Variable
n
Serotonin Glucuronidation (nmol/min/mg)
4-Nitrophenol Glucuronidation (nmol/min/mg)
UGT1A6 Protein (Relative to Lowest)
UGT1A6 mRNA (Relative to Lowest)
Median
(25–75%)
Median
(25–75%)
Median
(25–75%)
Median
(25–75%)
Gender
   Male 38 3.7 2.5–6.2 15 10–22 30 21–59 3.6 2.0–5.9
   Female 15 3 2.0–5.1 12 7–25 21 9–52 3.3 2.6–6.5
Smoking
   No 26 3.4 2.3–6.5 14 9–21 27 13–58 3.3 1.8–4.2
   Yes 18 5 2.5–7.6 19 11–25 37 28–57 5.0 2.4–8.4
Alcohol
   No 31 2.9*** 2.3–5.1 12* 9–22 28** 10–41 2.9* 1.8–5.2
   Yes 13 6.5*** 5.2–8.9 19* 15–30 57** 32–91 5.2* 3.4–9.0
- 1535 g→a
   g/g 33 3.7 2.3–6.2 16 10–25 32 20–56 3.4 1.8–6.2
   g/a 19 3.4 2.4–6.5 13 10–22 31 14–63 3.7 2.7–6.0
   a/a 1 2.8 11 11 4.2
- 1377 g→a
   g/g 23 2.9 2.3–5.6 14 10–22 30 16–53 3.9 2.5–7.0
   g/a 20 5.6 2.4–9.9 16 11–27 42 15–77 3.3 1.7–4.6
   a/a 10 3.4 2.6–6.4 11 10–24 29 21–48 3.8 1.7–6.0
- 427 g→c
   g/g 30 3.6 2.4–6.1 15 10–25 30 20–51 3.4 1.8–5.9
   g/c 21 3.6 2.4–6.1 12 9–21 31 15–62 3.7 2.6–6.5
   c/c 1 2.8 11 11 4.2
S7Aa
   S/S 23 3.0 2.4–6.0 14 10–22 30 16–53 3.9 2.5–7.0
   S/A 21 5.6 3.2–9.9 16 10–25 33 9.9–71 3.4 1.8–5.5
   A/A 9 3.6 2.5–6.9 15 9–24 34 27–56 2.4 1.7–6.2
T181A
   T/T 28 3.3 2.4–6.3 14 10–22 30 16–55 3.6 2.4–6.1
   T/A 17 5.4 2.6–8.4 14 11–23 45 10–72 3.2 1.8–5.5
   A/A 8 3.7 2.7–7.5 18 9–33 28 26–37 5.2 1.9–6.4
R184S
   R/R 27 3.1 2.3–6.5 14 10–23 29 16–56 3.7 2.5–6.2
   R/S 19 5.8 3.0–8.9 15 11–25 45 16–69 3.2 1.7–5.7
   S/S
7
3.6
2.5–5.8
14
9–23
28
26–40
3.8
1.7–6.5

  • Differences analyzed by Kruskal-Wallis ANOVA on rank transformed data except for demographic data that were analyzed by Mann-Whitney rank sum test (*p < 0.05; **p < 0.01; ***p < 0.001).

  • ↵ a Identical data were obtained for promoter polymorphisms -1710 c→g, -1306 to -1310 delete (aggag), and -652 g→a, which were in complete linkage disequilibrium with S7A