Primer and TaqMan probe sequences for the rat genes characterized in this experiment
Gene | Accession No. | Primers/Probes | Sequence* |
|---|---|---|---|
| GAPDH | NM_01008 | Forward primer | 5′ CCCCCAATGTATCCGTTGTG 3′ |
| Reverse primer | 5′ TAGCCCAGGATGCCCTTTAGT 3′ | ||
| TaqMan probe | 5′ TGCCGCCTGGAGAAACCTGCC 3′ | ||
| ITGAM | U59801 | Forward primer | 5′ CTGCCTCAGGGATCCGTAAAG 3′ |
| Reverse primer | 5′ CCTCTGCCTCAGGAATGACATC 3′ | ||
| TaqMan probe | 5′ CCCGGGACAATGCCGCGAA 3′ | ||
| GFAP | AF332062 | Forward primer | 5′ GAGCGTGCAGAGATGATGGAG 3′ |
| Reverse primer | 5′ TTCAGTTCAGCTGCCAGCG 3′ | ||
|
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| TaqMan probe | 5′ ATCGAGAAGGTCCGCTTCCTGGAACAG 3′ |
↵* The TaqMan probe has a reporter fluorescent dye, 6-carboxyfluorescein, at the 5′ end and fluorescence dye quencher, 6-carboxytetramethyl-rhodamine, at the 3′ end