cAMP accumulation data including pEC50, maximum values expressed as a percentage of 10 μ M forskolin stimulation [Max (%F)], and total cAMP accumulation expressed as picomoles produced per well (Max) for each relaxin family peptide tested at RXFP1, RXFP2, RXFP1/2, and RXFP2/1
Values are expressed as means ± the standard error of the mean of four experiments.
H2 Relaxin | Porcine Relaxin | Rhesus Monkey Relaxin | Rat Relaxin | INSL3 | ||
|---|---|---|---|---|---|---|
| RXFP1 | pEC50 | 9.57 ± 0.57 | 9.09 ± 0.18 | 9.76 ± 0.38 | 7.70 ± 0.10 | N.R. |
| Max (%F) | 19.52 ± 1.93 | 17.14 ± 3.84 | 17.67 ± 4.07 | 18.34 ± 3.99 | 0.04 ± 0.06 | |
| Max | 23.35 ± 4.22 | 29.64 ± 8.32 | 30.78 ± 7.74 | 31.55 ± 8.92 | 0.04 ± 0.07 | |
| RXFP2 | pEC50 | 7.72 ± 0.22*** | 8.05 ± 0.29*** | 5.40 ± 0.73*** | N.R. | 8.16 ± 0.27*** |
| Max (%F) | 15.68 ± 2.03* | 13.58 ± 5.17 | 10.28 ± 2.96 | N.R. | 15.58 ± 1.48*** | |
| Max | 19.23 ± 5.35 | 26.16 ± 11.42 | 12.73 ± 3.74* | 0.19 ± 0.01** | 13.93 ± 3.36*** | |
| RXFP1/2 | pEC50 | 9.20 ± 0.53 | 8.84 ± 0.17 | 8.91 ± 0.24** | 6.58 ± 0.16*** | 8.50 ± 0.89*** |
| Max (%F) | 17.11 ± 3.29 | 18.34 ± 2.56 | 16.98 ± 2.89 | 19.41 ± 3.55 | 3.49 ± 1.57* | |
| Max | 15.54 ± 3.89 | 17.39 ± 2.24 | 15.13 ± 3.23* | 17.87 ± 3.05 | 2.20 ± 0.29*** | |
| RXFP2/1 | pEC50 | 8.19 ± 0.15† | 8.03 ± 0.11 | 6.31 ± 0.50 | N.R. | 9.01 ± 0.54† |
| Max (%F) | 14.60 ± 4.05 | 15.22 ± 3.13 | 12.72 ± 4.87 | 0.17 ± 0.04††† | 11.69 ± 1.65† | |
|
| Max | 15.29 ± 2.72 | 16.85 ± 0.66 | 11.24 ± 3.34 | 1.33 ± 1.19 | 11.50 ± 2.24 |
N.R., no response.
↵* p < 0.05, ** p < 0.01, and *** p < 0.001 versus the related RXFP1 value (unpaired t test); †p < 0.05, and †††p < 0.001 versus the related RXFP2 value (unpaired t test).