Administration of URB597 (0.3 mg/kg i.p.) inhibits [3H]anandamide hydrolysis (picomoles per minute per milligram of protein) and elevates OEA and PEA levels (picomoles per gram) in rat liver The treatment has no significant effect on anandamide levels. Data are expressed as mean ± S.E.M. n = 8. FAE levels and [3H]anandamide hydrolysis in liver from rats treated with URB597.
Anandamide | OEA | PEA | [3H]Anandamide Hydrolysis | |
|---|---|---|---|---|
| Vehicle | 5.6 ± 0.6 | 21.1 ± 2.6 | 96.0 ± 6.6 | 1143 ± 31.8 |
| 30 min | 7.1 ± 0.6 | 80.6 ± 8.9** | 326.9 ± 22.4** | 212.2 ± 22.8** |
| 1 h | 6.5 ± 0.5 | 86.1 ± 5.5** | 339.3 ± 23.5** | 192.6 ± 28.4** |
| 2 h | 5.9 ± 0.7 | 68.8 ± 11.0** | 289.1 ± 25.3** | 271.7 ± 31.8** |
| 4 h | 5.6 ± 0.5 | 65.4 ± 5.2** | 245.7 ± 15.2** | 488.7 ± 40.4** |
| 8 h | 5.1 ± 0.3 | 31.7 ± 1.6 | 159.6 ± 20.1* | 1101 ± 6.1 |
| 16 h | 5.4 ± 0.7 | 26.9 ± 1.7 | 114.2 ± 7.6 | 1130 ± 7.3 |