Effects of CX546 on spontaneous miniature EPSCs recorded from RTN neurons and from hippocampal CA1 pyramidal cells All miniature events of a given experiment that satisfied the criteria described in the text were averaged and used to determine the mean amplitude, rise time, and decay time constant for that experiment. Data from n experiments (n = 5 or 10) were then averaged to obtain the mean and S.E.M. values shown in the table. Changes produced by CX546 were calculated for each experiment and averaged to obtain the values in the third row. The p value in the bottom row indicates the significance for the ratios in the third row being different between RTN and hippocampus (t test with Welch's correction for unequal variances; one-tailed).
RTN (n = 10) | Hippocampus (n = 5) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amplitude | 10–90% Rise Time | Decay τ | Frequency | Amplitude | 10–90% Rise Time | Decay τ | Frequency | |||||||
| pA | ms | Hz | pA | ms | Hz | |||||||||
| Control | 22.6 ± 2.4 | 1.4 ± 0.3 | 4.1 ± 0.5 | 3.8 ± 0.3 | 27 ± 7 | 1.6 ± 0.3 | 4.2 ± 0.9 | 2.9 ± 0.9 | ||||||
| CX546 (400 μM) | 25.1 ± 3.2 | 1.6 ± 0.3 | 5.8 ± 0.8*** | 3.8 ± 0.4 | 29 ± 11 | 3.5 ± 0.7* | 13.1 ± 4.1* | 2.9 ± 0.8 | ||||||
| Average ratio ± CX546 | 1.1 ± 0.1 | 1.2 ± 0.02 | 1.4 ± 0.1 | 1.0 ± 0.0 | 1.0 ± 0.1 | 2.6 ± 1.0 | 3.0 ± 0.7 | 1.1 ± 0.1 | ||||||
| Comparison hp vs. RTN |
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| N.S. | N.S. | p = 0.037 | N.S. | ||||||