Effects of signaling inhibitors on 10 μM forskolin-induced relaxation of rat bladder strips Data are means ± S.E.M. of 5 to 13 experiments and are expressed as a percentage of relaxation. ODQ was compared with dimethylsulphoxide, glibenclamide and H89 to ethanol, apamin to acetic acid, and all other inhibitors to deionized water. Note that the Rp-cAMPS experiments were done at a later point in time and hence were compared with a different control group (against passive, 39 ± 5% and against KCl, 74 ± 3%; n = 6 each).
Against Passive Tension | Against KCl-Induced Precontraction | |
|---|---|---|
| Absence of inhibitors | ||
| H2O | 37 ± 3 | 70 ± 2 |
| Ethanol | 38 ± 3 | 73 ± 1 |
| DMSO | 42 ± 4 | 70 ± 2 |
| Acetic acid | 37 ± 3 | 61 ± 6 |
| Cyclic nucleotide-dependent pathways | ||
| SQ 22,536 | 26 ± 3 | 74 ± 3 |
| H7 | 27 ± 3 | 73 ± 1 |
| H89 | 38 ± 5 | 66 ± 5 |
| Rp-cAMPS | 23 ± 3* | 75 ± 2 |
| ODQ | 30 ± 3 | 72 ± 3 |
| l-NNA | 29 ± 4 | 67 ± 3 |
| Potassium channels | ||
| BaCl2 | 44 ± 4 | 73 ± 2 |
| Tetraethylammonium | 35 ± 6 | 69 ± 4 |
| Glibenclamide | 35 ± 7 | 64 ± 2 |
| Apamin | 43 ± 4 | 63 ± 2 |
| Charybdotoxin | 45 ± 4 | 49 ± 2* |
| Iberiotoxin | 41 ± 6 | 56 ± 1* |
| Combinations | ||
| SQ + ODQ | 17 ± 3* | 59 ± 3* |
| SQ + charybdotoxin | 32 ± 3 | 46 ± 1* |
| ODQ + charybdotoxin | 41 ± 3 | 51 ± 1* |
| SQ + ODQ + charybdotoxin | 17 ± 3* | 49 ± 2* |
DMSO, dimethyl sulfoxide.
↵* P < 0.05 vs. corresponding vehicle control in a two-tailed t test or one-way analysis of variance followed by Dunnett's tests