Effects of PKC and Src inhibitors, alone or in combination, on the [3H]NE release induced by SRIF-14 + NMDA + glycine from rat hippocampal synaptosomes
SRIF-14 (1 nM), NMDA (100 μM), and glycine (1 μM) were added 8 min after the kinase antagonists. Results are expressed as percentage of increase over basal release. Data are means ± S.E.M. from five experiments run in triplicate.
[3H]NE Release | |
|---|---|
| SRIF-14 + NMDA + glycine | 72.97 ± 13.36 |
| SRIF-14 + NMDA + glycine + GF109203X (0.1 μM) | 29.84 ± 6.51a |
| SRIF-14 + NMDA + glycine + PP2 (1 μM) | 27.47 ± 6.73a |
| SRIF-14 + NMDA + glycine + GF109203X (0.1 μM) + PP2 (1 μM) | 28.36 ± 8.94a |
↵ a p < 0.05 vs. control.