TABLE 1

Ligand binding properties of α_1D-AR alone versus α_1D-/β₂-AR

HEK-293 cells were either transfected with α_1D-AR alone or cotransfected with α_1D-AR and β₂-AR. Specific binding of [¹²⁵I]-BE was measured as described under Materials and Methods. B_max and K_d values were calculated by nonlinear regression of saturation curves, and K_i values for inhibition of binding by BMY7378, prazosin, and NE were also determined. Each value represents the mean ± S.E.M. for three to six experiments in duplicate.