TABLE 2

Effect of BG on the catalytic activities, reduced CO difference spectra, and heme contents of the P450s

The values shown are the percentage of catalytic activity, reduced CO difference spectrum, and heme content remaining after the BG-dependent inactivation compared with the control samples incubated in the absence of NADPH. Experimental conditions were as described under Materials and Methods. The data shown represent the mean and S.D. values from three experiments for P450s 2B6 and 3A5. One experiment each was carried out for P450s 2B1, 2B4, and 3A4 for comparison purposes only.