Cell Type | pKIa | pKAb | pKBc |
---|---|---|---|
CHO high | 8.24 ± 0.07 (4) | 6.93 ± 0.48 (3) | N.D. |
+ GTPγS (10 μM) | 6.99 ± 0.14 (4) | ||
CHO Medium | 7.88 ± 0.39 (4) | 6.95 ± 0.48 (3) | 8.23 ± 0.11 (8) |
+ GTPγS (10 μM) | 7.51 ± 0.41 (4) | ||
CHO Low | 7.90 ± 0.33 (4) | 6.53 ± 0.33 (3) | 7.95 ± 0.15 (4) |
+ GTPγS (10 μM) | 6.93 ± 0.41 (4) | ||
FVB mouse ileum | 5.54 ± 0.28 (3) | N.D. | N.D. |
3T3-F442A | 5.79 ± 0.47 (3) | N.D. | 7.91 ± 0.33 (4) |
N.D., not determined.
↵ a Negative logarithm of the SR59230A dissociation constant as determined from [125I]cyanopindolol competition binding; b negative logarithm of the SR59230A dissociation constant as determined from operational model fitting of cAMP functional data (Fig. 1); c negative logarithm of the SR59230A apparent dissociation constant as determined using a single concentration of antagonist against CL316243-mediated changes in extracellular acidification rate (Figs. 3 and 6).