TABLE 2

Effect of MAPK inhibitors on the increase of heme oxygenase activity mediated by rosolic acid and 2′-hydroxychalcone Endothelial cells were preincubated for 15 min with 10 μM PD098059 (ERK inhibitor), 10 μM SB203580 (p38 inhibitor), or 10 μM SP600125 (JNK inhibitor) prior to exposure for 6 h to 15 μM RA or 15 μM 2′OH-CAL. Heme oxygenase activity was determined as described under Materials and Methods, and results are expressed as a percentage of the activity promoted by RA or 2′OH-CAL, respectively.

Heme Oxygenase Activity (pmol bilirubin/mg protein/h)
+ PD098059 + SB203580 + SP600125
%
RA 100 86 ± 0.01 100 ± 0.01 72 ± 0.17
2′OH-CAL 100 80 ± 0.02 85 ± 0.02 127 ± 0.05