Primers and probes for quantitative PCR
| Genes | Primer | Probe | |
|---|---|---|---|
| Forward | Reverse | ||
| PPARγ | CATGACCAGGGAGTTCCTCAA | AGCAAACTCAAACTTAGGCTCCAT | CCTGCGGAAGCCCTTTGGTGACT |
| EnaCα | GAGAGCCTGGCACAGAAAGG | CAGCTTTCACGCCCTCTTG | AGACCCAAAGAGAAGCGGGAGTCAGC |
| EnaCβ | TGTGTCTTCCAGCCTGACACA | CGGAATGGGCAGAGTCTGTT | TGCAGGCCCAATGCCGAGG |
| EnaCγ | CCTTCAAGCTGAGCGAACCT | GATGTTTGTGACGGGCACATC | ACAGCCAGTGCACAGAGGACGGC |
| GR | GGGACCACCTCCCAAGCT | GCACCCCGTAATGACATCCT | TGCCTGGTGTGCTCCGATGAAGC |
| MR | TCCATTACGCATGCACTCACA | CGGTCTCAGGCTCGATGTTC | CATCCCCGGCCATGATCCTGG |
| NPR-A | ATCACAGTGAATCACCAGGAGTTC | ACGGCCCGCAGTAGCTT | AGGGCGACCCGGACCACTACCC |
| Na,K-ATPase-α | GGCTGTCATCTTCCTCATTGG | CGGTGGCCAGCAAACC | ATCATCGTAGCCAACGTGCCGGA |
| AQP3 | ACCTCCATGGGCTTCAATTCT | AGTGAAAAGGCGAGGTCCAA | CTATGCCGTCAACCCAGCTCGTGA |
| AQP2 | CCCTCTCCATTGGTTTCTCTGT | GGCTGGATTCATGGAGCAA | CCTGGGCCACCTCCTTGGGATTATT |
| 11β-HSD1 | TGGAAGACATGGCTTTTGCA | TCCAGTCCACCCAAGAGCTT | AGCGATTTGTTGTTGAGGCAGG |
| 11β-HSD2 | GGCAAGGAGACAGCTAAGAAACTG | GGGCCATTCAAATCCAACAC | TGCCATGGGCTTCACGGTGC |
| SGK1 | GAGGGAGCGCTGCTTCCT | ACCCAAGGCACTGGCTATTTC | ACCCCGTGCTCGCTTCTACGCA |
| AVP-R2 | CCTGGGCCTTCTCACTCCTT | GCCATTTCCCACATCACGCT | TCAGCCTGCCTCAGCTCTTCATCTTTG |