TABLE 2

Dissociation constants of 4-DAMP and atropine estimated in contractile assays on the ileum Mean estimates ± S.E.M. are shown and were calculated from the data presented in Fig. 3.

Assay Ileum
Observed pKBa Resultant pKBb
Contraction
   Atropine (10 nM) 9.04 ± 0.071 NA
   4-DAMP (3 nM) 9.17 ± 0.12 9.04 ± 0.16
   4-DAMP (10 nM) 9.09 ± 0.14 9.15 ± 0.28

  • NA, not applicable.

  • ↵ a Negative logarithm of the observed KB value calculated according to eq. 1

  • ↵ b Negative logarithm of the observed KB value calculated according to eq. 2