Sequences of primers, hybridization probes, annealing temperature, and lengths of PCR products
| DNMT1 | 332-bp product | XM_017218a | 62°C |
| DNMT 1 S | 5′-gCT TCT ACT TCC TCg Agg CCT A | ||
| DNMT 1 A | 5′-gTT gCA gTCCTCTgTgAACACTgTg | ||
| hyb DNMT1 LC | 5′-LC Red640-CTg Tgg gCC ATC gAg Atg Tgg gAC p | ||
| hyb DNMT1 FL | 5′-TTC CAC CAA gCA ggC ATC TCT gAC AC X | ||
| DNMT3a | 428-bp product | AF_067972 | 62°C |
| Met 3a F 1 | 5′-gTC ACA CAg AAg CAT ATC CAg gAg Tg | ||
| Met 3a R 1 | 5′-gTC CTC ACT TTg CTg AAC TTg GC | ||
| hyb DNMT3a FL | 5′-ACg Atg gAg Agg TCA TTg CAg ggA C X | ||
| hyb DNMT3a LC | 5′-LC Red640-gCC CCC AAT CAC CAg ATC gAA Tg p | ||
| DNMT3b | 191-bp product | XM_009449 | 62°C |
| Meth3b-F | 5′-AAT gTg AAT CCA gCC Agg AAA ggC | ||
| Meth3b-R | 5′-ACT ggA TTA CAC TCC Agg AAC CgT | ||
| hyb DNMT3b FL | 5′-ggC gTg AgT AAT TCA gCA ggT ggT X | ||
| hyb DNMT3b LC | 5′-LC Red640-ATT CgA AgA AgA gCC ggC CTg TAC C p | ||
| GADD45 | 212-bp product | NM_001924 | 57°C |
| GADD45F | 5′-CTg CgT gCT ggT gAC gAA | ||
| GADD45A | 5′-Agg gAg TAA CTg CTT gAg TAA CTA | ||
| hyb GADD45 FL | 5′-TCA ggg AgA TTA ATC ACT ggA ACC C X | ||
| hyb GADD45 LC | 5′-LC Red640-TTG ATC CAT gTA gCg ACT TTC CCg-PH | ||
| p16 | 127-bp product | NM_000077 | 63°C |
| P16 F | 5′-Tgg ACC Tgg CTg Agg AgC T | ||
| P16 A | 5′-TCA ATC ggg gAT gTC TgA g | ||
| hyb p16 FL | 5′-gCg gCA TCT Atg Cgg gCA Tgg TT X | ||
| hyb p16 LC | 5′-LC Red640-CTg CCT CTg gTg CCC CCC gC p |
LC, LightCycler Red 640; X, fluorescein; p, phosphate; hyb, hybridization probe.
↵ a GenBank accession number