DOX-mediated induction of PGE2 release in three patient samples
Cells were incubated for 72 h with or without 0.01 μg/ml DOX. PGE2 concentrations were subsequently determined in culture supernatants by a PGE2-specific competitive ELISA. Data represent mean values of three independent culture bottles per culture condition, each measured in duplicate.
PGE2 Release after 72h | ||||
|---|---|---|---|---|
| Collection ID | DOX = 0 μg/ml | DOX = 0.01 μg/ml | ||
| pg/ml | ||||
| 1 | 447 | 853 | ||
| 2 | 415 | 886 | ||
| 3 | 1187 | 8044 | ||
Each DOX-treated sample significantly different from untreated controls, ***p < 0.001; Student's t test for unpaired values.