TABLE 2

EC50 values and maximal effects of salvinorin A, TRK-820, and 3FLB in stimulating [35S]GTPγS binding to membranes of CHO cells stably expressing the μ, δ, and κ opioid or N/OFQ receptors

DAMGO, DPDPE, U50,488H, and N/OFQ were used as the full agonists for the μ, δ, and κ opioid and N/OFQ receptors, respectively. Data were derived from the curves in Fig. 3.



μ

δ

κ

N/OFQ

EC50
Maximal Effect
EC50
Maximal Effect
EC50
Maximal Effect
EC50
Maximal Effect
nM % nM % nM % nM %
DAMGO 8.9 ± 2.1 100
DPDPE 3.4 ± 0.7 100
U50,488H 2.2 ± 0.3 100
Nociceptin/OFQ 1.2 ± 0.2 100
TRK-820 3.2 ± 1.3 54 ± 7 289 ± 60 51 ± 6 0.025 ± 0.003 93 ± 5 147 ± 30 27 ± 4
Salvinorin A N.S. N.S. 4.6 ± 1.2 110 ± 12 N.S.
3FLB
N.S.

N.S.

73.6 ± 12
90 ± 5
N.S.

  • N.S., no stimulation up to 1 μM.