TABLE 1

Apparent Ki values (nanomolar) of salvinorin A, TRK-820, 3FLB, and U50,488H for the opioid receptors and the N/OFQ receptor in [35S]GTPγS binding buffer

Ki values were calculated from the equation Ki = IC50/(1 + [L]/Kd) (Cheng and Prusoff, 1973). IC50 values were derived from the competition curves shown in Fig. 2.

μ
δ
κ
N/OFQ
TRK-820 5.2 ± 0.8 161 ± 42 0.075 ± 0.007 N.A.
Salvinorin A N.A. N.A. 7.9 ± 0.8 N.A.
3FLB N.A. N.A. 248 ± 23 N.A.
U50,488H
N.A.
N.A.
11.0 ± 0.2
N.A.

  • N.A., did not bind at 1 μM.