Representative enrichment of various efflux transporters in plasma membrane preparation compared with whole cell lysates from BBMECs
Cellular Fraction | Enrichment* | ||||||
|---|---|---|---|---|---|---|---|
| P-gp | MRP1 | MRP5 | MRP4 | ||||
| Total membrane | 38 | 9 | 5 | 18 | |||
| Apical (F1) | 134 | 32 | 16 | 88 | |||
| Basolateral (F3) | 12 | 10 | 9 | 60 | |||
↵* Enrichment of the various membrane fractions compared with whole cell lysates was determined using densitometry of the immunoblots from one representative membrane isolation.