TABLE 1

Influence of RPPGF derivatives on γ -thrombin-induced platelet aggregation and calcium mobilization

Data presented are the mean ± S.E.M. of n > 3 independent experiments. In all instances, the values given represent the concentration of peptide needed to inhibit threshold γ -thrombin-induced platelet aggregation 100%. Calcium mobilization assays were performed as described under Materials and Methods. In all instances, the values given represent the influence of 100 mM peptide on threshold α-thrombin-induced calcium mobilization.

Peptide
Sequence
Platelet Aggregation 100% Inhibitory Conc.
Calcium Mobilization
mM ± S.E.M. Percentage of inhibition at 100 mM peptide
RPPGFa RPPGF 0.68 ± 0.28
TH146 ROicPGF 0.142 ± 0.02 54
MAP4-TH146b 0.019 ± 0.006 80
TH34 ROicPGIdg 0.129 ± 0.02 36.2
TH37 RoicHypGThi 0.157 ± 0.02 38
TH40 ROicHypGIdg 0.157 ± 0.02 36.7
TH26 ROicPGThi 0.228 ± 0.02 14.5
TH46 ROicOicGIdg 0.257 ± 0.04 28.1
TH43
ROicOicGThi
0.271 ± 0.04
18.1

  • ↵ a From Hasan et al. (2001a).

  • ↵ b MAP4-TH146 is a multiantigenic peptide of TH146 consisting of β-AK2K-4(rOicPGF).