Curve fit parameters for antagonism of 5-HT (10 nM)-stimulated calcium signaling in HEK-293 cells expressing 5-HT2C-GFP Cells were preincubated for 30 min in the presence of the indicated inverse agonists and neutral antagonists and subsequently challenged with 10 nM 5-HT to assess antagonism. n is the number of independent experiments used to determine the mean ± S.E.M.