TABLE 3

Regiochemistry and stereochemistry of arachidonic acid metabolites produced by CYP2C44 Recombinant, partially purified CYP2C44 was reconstituted with cytochrome P450 reductase and phospholipids, and incubated with [1-¹⁴C]AA as described under Materials and Methods. The regiochemical distribution of EETs and HETEs was quantified by HPLC and liquid scintillation as described under Materials and Methods. Values were representative of four separate experiments.