TABLE 2

Ability of hBRS-3-selective analogs [d-Tyr⁶, (R)-Apa¹¹-4Cl, Phe¹³, Nle¹⁴]Bn(6-14) (peptide 7) and the nonselective ligand [d-Tyr⁶, β-Ala¹¹, Phe¹³, Nle¹⁴]Bn(6-14) (peptide 1) to interact with various gastrointestinal hormone/neurotransmitter receptors

AR42J cells (1 × 10⁶ cells/ml), dispersed rat acini (1 pancreas/10 ml), and CHO cells that were stably transfected with hVPAC₁-R (0.2 × 10⁶ cells/ml) or hVPAC₂-R (3.0 × 10⁶ cells/ml), were incubated with 50 pM of the indicated ¹²⁵I-ligand or 0.6 nM of [N-methyl-³H]scopolamine methyl chloride for 60 min as specified under Materials and Methods. Results are the percentage of the total saturable binding with or without 1 μM peptide 1 or peptide 7 (Table 1). All results are the means ± S.E.M. from at least four experiments, and in each experiment each value was performed in duplicate.