Relative affinity of SNC-80 and enkephalins for hDOR in SK-N-BE cells
Ki1 and Ki2 values, corresponding to high- and low-affinity sites, respectively, were determined from competition of [3H]diprenorphine binding by SNC-80, Leu-, and Met-enkephalin. Data are means ± S.E.M. from three different experiments performed in triplicate.
Agonist | Binding Ki | Percentage of Each Site |
|---|---|---|
| nM | ||
| SNC-80 | Ki1 = 0.10 ± 0.01 | 54.5 ± 5.7 |
| Ki2 = 54.1 ± 19.5 | 45.5 ± 4.3 | |
| Leu-enkephalin | Ki1 = 0.18 ± 0.03 | 63.4 ± 5.1 |
| Ki2 = 54.4 ± 9.0 | 36.6 ± 4.9 | |
| Met-enkephalin | Ki1 = 1.01 ± 0.18* | 74.9 ± 5.2 |
|
| Ki2 = 115 ± 34 | 25.1 ± 4.8 |
↵* Significantly different from SNC-80 and Leu-enkephalin; p < 0.01, ANOVA followed by Bonferroni-Dunn test.