TABLE 3

ESI-LC-MS/MS analysis of adduct B from tBA-inactivated P450s 2E1 and 2E1 T303A under nonacidic solvent conditions P450s 2E1 and 2E1 T303A were reconstituted with reductase and lipid as described under Materials and Methods. Control and tBA-inactivated P450 sample components were resolved by reverse phase chromatography under nonacidic solvent conditions (water, acetonitrile). In some cases, 0.1% TFA was added to preacidify the sample before injection. MS/MS analysis of the tBA-modified heme products (m/z of 661 Da) was performed with 0.4 m/z isolation width and 25% collision energy. The values shown are representative of data obtained from three independent experiments.