TABLE 2

Effect of AS601245 in LPS-induced TNF-α production in mice C3H/HEN mice were pretreated with AS601245 (0.3, 1, 3, and 10 mg/kg p.o.). Fifteen minutes later, LPS (0.3 mg/kg) was administered i.p. After retro orbital puncture, the whole blood was collected, and the plasma was analyzed for TNF-α levels by enzyme-linked immunosorbent assay. Data are mean ± S.E.M.


Treatment, mg/kg p.o.

TNF-α

Inhibition

p <
pg/ml %
Control LPS 3383 ± 105
Dexamethasone, 0.1 417 ± 67 88 ± 2 0.001
AS601245, 0.3 2483 ± 213 27 ± 6 0.001
AS601245, 1 2022 ± 50 40 ± 1 0.001
AS601245, 3 1719 ± 120 49 ± 4 0.001
AS601245, 10
1317 ± 73
61 ± 2
0.001