TABLE 4
The quantification of CYP3A forms in liver and small intestine of male and female rats. Immunoblot analysis was carried out as described under Materials and Methods. The numbers represent the ratio of CYP3A form to microsomal protein (pmol/mg protein). The value represents the mean and the standard deviation of four different rats. <0.10, less than 0.10 pmol/mg protein in liver, and <0.02, less than 0.02 pmol/mg protein in small intestine. The value of CYP3A1 and CYP3A2 forms in male liver represents the total amount of both CYP3A1 and CYP3A2 due to incomplete separation. *, CYP3A62 and CYP3A18 forms were separated incompletely in SDS-PAGE, but the stained band could be divided into two upper (CYP3A62) and lower (CYP3A18) portions by using NIH image 1.59/ppc.