Translocation of γPKC in lamina II from L5 spinal cord from P7, P15, or P21 rats after intraplantar formalin An increase in γPKC-ir in lumbar spinal cord after intraplantar formalin compared with intraplantar saline or normal naïve tissue was indicative of translocation. Pretreatment with ϵV1-2 or γV5-3 (50 pmol/5 μl, intrathecal 15 min before formalin), but not Tat carrier, blocked the change in γPKC-ir in lumbar spinal cord. Data represented as average ± S.D.
Age (days) | Normal | 10-min Formalin | 10-min Formalin + Tat Carrier | 10-min Formalin + γV5-3 | 10-min Formalin + ϵV1-2 | 10-min Saline |
|---|---|---|---|---|---|---|
| days | ||||||
| 7 | 100 ± 3.4 | 98.5 ± 4.1 | 105 ± 4.5 | 102 ± 5.3 | 95.9 ± 6.2 | 102 ± 5.6 |
| 15 | 100 ± 5.6 | 112 ± 7.6 | 116 ± 8.2 | 105 ± 6.4 | 107 ± 4.0 | 95.6 ± 4.6 |
| 21 | 100 ± 5.2 | 128 ± 6.5* | 132 ± 5.8* | 106 ± 4.8† | 103 ± 5.9† | 104 ± 3.5¶ |