TABLE 2

Effects of lysosphingolipid analogs on leukemic cell survival Cell survival was evaluated along multiple indices as outlined under Materials and Methods. Apoptotic DNA degradation was assessed by fluorescent microscopy, and apoptotic cytoarchitecture was assessed by light microscopy. Clonogenic growth was monitored by soft agar assay for colony formation. All values reflect the mean ± standard error for triplicate determinations. Data shown are from a representative study performed at least three times with comparable outcomes.


Treatmenta

Apoptotic DNA Damage

Apoptotic Cell Death

Treatmentb

Apoptotic DNA Damage

Apoptotic Cell Death

Clonogenicity
% total % total % control
Vehicle 4 ± 1 2 ± 1 Vehicle 3 ± 0 2 ± 1 100 ± 2
So 87 ± 6 74 ± 7 Sa 82 ± 3 74 ± 3 2 ± 1
Sa 84 ± 4 75 ± 3 PEITC-Sa 91 ± 6 80 ± 4 1 ± 0
PEITC-So 93 ± 4 88 ± 6 PEITC 5 ± 2 3 ± 2 92 ± 7
PEITC-Sa
95 ± 7
84 ± 8




  • a HL-60 cells were exposed to sphingosine (So), sphinganine (Sa), the corresponding PEITC-substituted analogs (PEITC-So and PEITC-Sa), or vehicle for 6 h

  • b HL-60 cells were exposed to PEITC-Sa, free Sa, free PEITC, or vehicle for 6 h