TABLE 1

Acute actions of lysosphingolipid analogs on lipid-driven PKC activity Membranal cPKC/nPKC activity was isolated and assayed in vitro as described under Materials and Methods. Values reflect the mean ± standard error for quadruplicate determinations. Data shown are from a representative study performed at least three times with comparable outcomes.

Treatment^a	cPKC/nPKC Activity	Treatment^b	cPKC/nPKC Activity
	% untreated control		% untreated control
Vehicle	100 ± 1	Vehicle	100 ± 3
Sphingosine	7 ± 1	PEITC-Sa	6 ± 2
Sphinganine	4 ± 4	Sphinganine	6 ± 3
PEITC-So	3 ± 2	PEITC	97 ± 5
PEITC-Sa

↵ a HL-60 cells were exposed to sphingosine (So), sphinganine (Sa), and the corresponding PEITC-substituted analogs (PEITC-So and PEITC-Sa), or vehicle for 30 min
↵ b HL-60 cells were exposed to PEITC-Sa, free Sa, free PEITC, or vehicle for 30 min