IC50 value of Cdc25 inhibitors for different human tumor cell lines Cytotoxicity was determined by colony formation assay as described in the text. IC50 values, representing mean ± S.D. of three separate experiments, were calculated using a Sigmoid-Emax model as implemented by the ADAPT II computer program.
Cell Lines | IC50 | ||||
|---|---|---|---|---|---|
| Cpd 5 | NSC 95397 | NSC 663284 | |||
| μM | |||||
| HCT-116 | 2.3 ± 0.3 | 1.3 ± 0.1 | 2.3 ± 0.2 | ||
| HCT-116R30A | 0.4 ± 0.1 | 1.1 ± 0.1 | 2.4 ± 0.2 | ||
| HCT-116R30A/NQ5 | 1.7 ± 0.2 | 1.3 ± 0.1 | 2.1 ± 0.3 | ||
| MDA-MB-231/Q2 | 0.3 ± 0.1 | 0.9 ± 0.1 | 3.2 ± 0.4 | ||
| MDA-MB-231/Q6 | 0.7 ± 0.1 | 1.1 ± 0.1 | 2.9 ± 0.5 | ||
| HT-29 | 6.7 ± 0.3 | 1.8 ± 0.3 | 2.7 ± 0.3 | ||