Maximum responses and pEC50 values for phenylephrine and methoxamine-induced ERK1/2 phosphorylation in human recombinant α1-ARs stably expressed in HEK 293 cells
Each value is the mean ± S.E.M. of three independent experiments.
Agonist | α1A | α1B | α1A,1B |
|---|---|---|---|
| Phenylephrine | |||
| pEC50 | 6.8 ± 0.1 | 6.7 ± 0.2 | 7.6 ± 0.2* |
| Max. response | 6.9 ± 1.8 | 7.4 ± 0.9 | 20.2 ± 3.3* |
| Methoxamine | |||
| pEC50 | 6.7 ± 0.1 | 4.8 ± 0.1* | 6.7 ± 0.1 |
| Max. response | 8.6 ± 1.8 | 4.9 ± 1.5* | 7.5 ± 1.1 |
Max. response, maximum ERK1/2 activation induced by phenylephrine or methoxamine (fold over basal); ND, not determined.
↵* p < 0.05 significantly different from the values of other cell lines.