Effect of 2-h preincubation (or not) of isolated human fat cells with isoproterenol (0.1 μmol/l) or ANP (0.01 μmol/l) on the lipolytic response induced by isoproterenol or ANP
EC50 and pD2 values were calculated from concentration-response curves of isoproterenol (10-10 to 10-5 M) and of ANP (10-11 to 10-6 M). Values are means ± S.E.M. of six separate experiments. P values were obtained when comparing values obtained with preincubated cells with those from control cells.
EC50 | pD2 | |
|---|---|---|
| nmol/l | ||
| Control cells | ||
| Isoproterenol | 14.2 ± 0.50 | 8.02 ± 0.2 |
| ANP | 0.62 ± 0.32 | 9.45 ± 0.20 |
| Cells preincubated with isoproterenol | ||
| Isoproterenol | 70 ± 0.22 (P < 0.01) | 7.30 ± 0.18 (P < 0.002) |
| ANP | 1.5 ± 0.82 (NS) | 9.35 ± 0.38 (NS) |
| Cells preincubated with ANP | ||
| Isoproterenol | 24 ± 0.8 (NS) | 7.79 ± 0.18 (NS) |
| ANP | 2.6 ± 0.65 (P < 0.05) | 8.69 ± 0.15 (P < 0.01) |