Summary of in vitro HCY formation in liver microsomes, CEPM formation in liver cytosol, liver cytosolic GST activity, and in vivo CEPM elimination in untreated TR- and untreated Wistar rats
All activities were measured as described under Materials and Methods.
Wistar | TR- | p Valuea | ||
|---|---|---|---|---|
| HCY formation (nmol/min/mg microsomal protein) | 0.1 mM CY | 0.74 ± 0.62 | 0.78 ± 0.50 | 0.90 |
| 0.5 mM CY | 2.00 ± 0.03 | 2.06 ± 0.1 | 0.60 | |
| 2 mM CY | 4.86 ± 0.32 | 4.98 ± 0.78 | 0.86 | |
| CEPM formation (nmol/min/mg cytosolic protein) | 5 μM HCY | 0.4 ± 0.054 | 0.444 ± 0.027 | 0.24 |
| 50 μM HCY | 3.34 ± 0.429 | 4.04 ± 0.325 | 0.06 | |
| GST activity (nmol/min/mg cytosolic protein) | 1 mM CDNB | 662 ± 61.46 | 623 ± 27.99 | 0.37 |
| CEPM elimination (ml/min) | Biliary clearance | 0.88 ± 0.22 | 0.29 ± 0.12 | 0.003 |
| Renal clearance | 7.08 ± 3.88 | 8.88 ± 2.38 | 0.84 | |
|
| Renal + biliary clearance | 7.58 ± 4.51 | 9.17 ± 2.46 | 0.56 |
↵ a p Value was determined by Student's t test between TR- and Wistar rat.