Primers, annealing temperatures, and cycle numbers for semiquantitative PCR
Primer | Sequence | Fragment Size | Annealing | Cycles |
|---|---|---|---|---|
| base pairs | °C | |||
| iNOS | ||||
| Antisense | CACCACAAGGCCACATCGGATT | 426 | 60 | 28 |
| Sense | CCGACCTGATGTTGCCATTGTT | |||
| IL-6 | ||||
| Antisense | CTGGTGACAACCACGGCCTCCCCT | 600 | 62 | 26 |
| Sense | ATGCTTAGGCATAACGCACTAGGT | |||
| IL-10 | ||||
| Antisense | CCTGGTAGAAGTGATGCCCCAGGCA | 237 | 55 | 30 |
| Sense | CTATGCAGTTGATGAAGATGTCAAA | |||
| 18S | ||||
| Antisense | CCCGGGGAGGTAGTGACGAAAAAT | 209 | 58 | 22 |
| Sense | CGCCCGCTCCCAAGATCCAACTAC |
|
|
|