Primers used for sequencing each CYP1A2 exon Numbers of bases upstream of exon 1 to 7 are 170 (Ex1F1), 80 (Ex2F1), 190 (Ex3F1), 130 (Ex4F1), 200 (Ex5F1), 120 (Ex6F1), and 100 (Ex7F1). Numbers of bases downstream of exon 1 to 7 are 60 (Ex1R1), 140 (Ex2R2), 170 (Ex3R1), 240 (Ex4R1), 140 (Ex5R1), 190 (Ex6R1), 50 (Ex7R1), and 240 (Ex7R2s). Thus, the above-mentioned primers used were designed in intronic regions surrounding each of exon 1 to 7 by the aid of OLIGO software (Molecular Biology Insights, Inc. West Cascade, CO).
Primer Name | Primer Sequence (5′ to 3′) |
|---|---|
| 1A2Ex1F1 | CTATCCCCAAAGAGTCACCC |
| 1A2Ex1R1 | CTCCCCGTCTTTCTGTCCC |
| 1A2Ex1F2 | CTTGACTTCTGTTTCCCGAT |
| 1A2Ex1R2 | CAATGCCATCTGTACCAACTG |
| 1A2Ex1F3 | AAGGTATCAGCAGAAAGCCA |
| 1A2Ex1R3 | CAGCACCCTTGTCAGCC |
| 1A2Ex2F1 | CAAGCACAGAACACGCATC |
| 1A2Ex2R1 | CAGAGAAGGGGAAGAGTCGG |
| 1A2Ex2F2 | CGCACCTGGCACTGTCA |
| 1A2Ex2R2 | CAATCAGGTGGTGGTGTCAG |
| 1A2Ex2F3 | CATCGCTACTCTCAGGGAA |
| 1A2Ex2R3 | GAAATCGTCGCCCTGCC |
| 1A2Ex3F1 | TGTTATTGGGAGGAAGGGTCA |
| 1A2Ex3R1 | GACTTTGCTGTTTCTCCACTG |
| 1A2Ex4F1 | TATGTTGAAGAGACCCAGCCT |
| 1A2Ex4R1 | TAGAACACAGAGGACAAGCAGA |
| 1A2Ex5F1 | CCCAGGTTGTTTGTTCAGTC |
| 1A2Ex5R1 | GGGGGTTAGATTATGTTTGGA |
| 1A2Ex6F1 | TTTCTACCTCTTCCCTGTTCC |
| 1A2Ex6R1 | AACTGCTGGGATTATAGGCTT |
| 1A2Ex7F1 | CTTCCCACCTACCCTTCATT |
| 1A2Ex7R1 | TGGGGTCTTGCTCTGTCACT |
| 1A2Ex7R2s | GGAAGAGAAACAAGGGCTGA |